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G9785

Sigma

 

GSEM Supplement

50 ×, liquid, sterile-filtered, cell culture tested

Synonym:Glutamine synthetase expression medium supplement
MDL number:MFCD00677096

Description

ApplicationThe glutamine synthetase (GS) system is used to express recombinant proteins from mammalian cells. Glutamine-dependent cells transfected with a glutamine synthetase gene can be selectively grown in a suitable glutamine-deficient medium.
PackagingContains (mg/L): L-alanine 450.0, L-asparagine ยท H2O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0
QuantityOne mL will supplement 50 mL of cell culture medium.

Properties

sterilitysterile-filtered
formliquid
concentration50 ×
total impurities Endotoxin, tested
suitabilitycell culture tested
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3

Related Products

Related productG6264, Guanosine
 S4311, L-Serine
 G8790, Glycine
 C4654, Cytidine
 T1895, Thymidine
 U3003, Uridine
 S4500, L-Serine
 A4036, Adenosine

References

referenceDoyle, A., et al. Cell and Tissue Culture: Laboratory Procedures Chichester, UK , (1999) 27D, 3.5-3.7